A sequence-tagged genetic map for the brown alga Ectocarpus siliculosus provides large-scale assembly of the genome sequence
Résumé
Ectocarpus siliculosus has been proposed as a genetic and genomic model for the brown algae and the 214 Mbp genome of this organism has been sequenced. The aim of this project was to obtain a chromosome-scale view of the genome by constructing a genetic map using microsatellite markers that were designed based on the sequence supercontigs.
To map genetic markers, a segregating F 2 population was generated from a cross between the sequenced strain (Ec 32) and a compatible strain from northern Chile. Amplified fragment length polymorphism (AFLP) analysis indicated a significant degree of polymorphism (41%) between the genomes of these two parental strains. Of 1,152 microsatellite markers that were selected for analysis based on their location on long supercontigs, their potential as markers and their predicted ability to amplify a single genomic locus, 407 were found to be polymorphic.
A genetic map was constructed using 406 markers, resulting in 34 linkage groups. The 406 markers anchor 325 of the longest supercontigs on to the map, representing 70.1% of the genome sequence.
The Ectocarpus genetic map described here not only provides a large-scale assembly of the genome sequence, but also represents an important tool for future genetic analysis using this organism.
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Genetic map paper 8Mar10.pdf (318.87 Ko)
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Fig.1 part1.tif (117.52 Ko)
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Fig.1 part2.tif (106.38 Ko)
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Fig.1 part3.tif (121.83 Ko)
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Fig.1 part4.tif (74.47 Ko)
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NPH_3273_Tables S2 and S3.xls (479 Ko)
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NPH_3273_sm_tS1.pdf (32.29 Ko)
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NPH_3273_sm_tS2-S3.xls (479 Ko)
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